一、什么是超凈工作臺呢？
超凈工作臺是一種局部凈化設(shè)備，即利用空氣凈化技術(shù)使一定操作區(qū)內(nèi)的空間達(dá)到相對的無塵、無菌狀態(tài)。使用時，必須放置在潔凈的空間。其接種數(shù)量不受無菌空的限制，操作比較簡便，可以較大幅度地提高工作效率，適用于大規(guī)模生產(chǎn)。為了保證接種成功率，接種時使用酒精燈，效果更好。
Clean Bench is a partial purification equipment, namely the use of air purification technology to a certain region of space operations to achieve a relatively dust-free, sterile condition. Used, must be placed in a clean space. The number of its vaccination is not sterile space constraints, the operation is simpler, can be more greatly improve work efficiency, suitable for mass production. In order to ensure the success rate of vaccination, inoculation using alcohol lamp, better.
二、超凈工作臺運(yùn)作原理及應(yīng)用方法
超凈工作臺的優(yōu)點(diǎn)是操作簡單自如，較為舒適，工作效率高，預(yù)備時間短，開機(jī)十分分鐘以上即可操作，基本上可隨時使用。在工廠化生產(chǎn)中，接種工作量很大，需要經(jīng)常長久地工作時，超凈臺是很理想的設(shè)備。超凈臺由三相電機(jī)作鼓風(fēng)動力，功率145～260W左右，將空氣通過由特制的微孔泡沫塑料片層疊合組成的“超級濾清器”后吹送出來，形成連續(xù)不斷的無塵無菌的超凈空氣層流，即所謂“高效的特殊空氣”，它除去了大于0.3μm的塵埃、真菌和細(xì)菌孢子等等。超凈空氣的流速為24～30m/min，這已足夠防止附近空氣可能襲擾而引起的污染，這樣的流速也不會妨礙采用酒精燈或本生燈對器械等的灼燒消毒。工作人員就在這樣的無菌條件下操作，保持無菌材料在轉(zhuǎn)移接種過程中不受污染。但是萬一操作中途遇到停電，暴露在未過濾空氣中的材料便難以幸免污染。這時應(yīng)迅速結(jié)束工作，并在瓶上作出記號，內(nèi)中的材料如處于增殖階段，則以后不再用作增殖而轉(zhuǎn)入生根培養(yǎng)。如為一般性生產(chǎn)材料，因極其豐富也可棄去。如處于生根過程，則可留待以后種植用。
Clean Bench has the advantage of easy to operate freely, more comfortable, efficient, preparation time is short, the boot can be operated for more than a very minute, basically ready to use. In factory production, inoculation heavy workload, the need for frequent long-term work, the Clean Bench is the ideal equipment. Clean Bench by the three-phase motor to make blast power, power 145 ~ 260W or so, will air through the porous foam by a specially composed piece cascading together a "super filter" after blowing out the formation of a continuous dust-free The ultra-clean air laminar flow bacteria, so-called "high-performance special air", which removed more than 0.3μm dust, fungi and bacteria spores and so on. Ultra-clean air flow rate of 24 ~ 30m/min, which are adequate to prevent possible harassment in the vicinity of air pollution caused by such a flow will not impede the use of alcohol lamp or Bunsen burner burning disinfection of equipment and so on. And in this way the staff operated under sterile conditions to maintain sterile material in the course of the transfer of vaccination is not contaminated. But what if an electrical outage halfway operation, exposed to unfiltered air contaminated material will not be spared. Should provide a speedy end to work and make a mark on the bottle, which is in the materials, such as the proliferation phase, we will no longer be used as a proliferation and transferred to rooting culture. As for the general production of materials, due to the extremely rich can also be disposable. As in the rooting process, can be planted until later use.
超凈工作臺電源多采用三相四線制，其中有一零線，連通機(jī)器外殼，應(yīng)接牢在地線上，另外三線都是相線，工作電壓是380V。三線接入電路中有一定的順序，如線頭接錯了，風(fēng)機(jī)會反轉(zhuǎn)，這時聲音正常或稍不正常，超凈臺正面無風(fēng)(可用酒精燈火焰觀察動靜，不宜久試)，應(yīng)及時切斷電源，只要將其中任何兩相的線頭交換一下位置再接上，就可解決。三相線如只接入兩相，或三相中有一相接觸不良，則機(jī)器聲音很不正常，應(yīng)立即切斷電源仔細(xì)檢修，否則會燒毀電機(jī)。這些常識應(yīng)在開始使用超凈臺時就向工作人員講解清楚，免除不應(yīng)造成的事故與損失。
Clean Bench power supply to use more three-phase four-wire system, of which 10 lines, connecting the machine casing should then firmly on the ground line, the other three lines are the phase, the working voltage is 380V. Third-line access to the circuit in a certain order, such as pick the wrong end of a thread, wind the opportunity to reverse, when the sound normal or slightly abnormal, Clean Bench front without wind (available alcohol lamp flame observation of static and dynamic, not long-trial), should be promptly cut off the power, as long as any of them as two-phase end of a thread to exchange position were followed, the will be over. Three-phase line, such as access only to two-phase or three-phase there is a similar connection is bad, then the machine sounds very normal, should be immediately cut off the power supply careful maintenance, otherwise they will burn motor. These common sense should be when they start using Clean Bench to explain clearly to staff, should not result in exemption from the accident and loss.
超凈工作臺進(jìn)風(fēng)口在背面或正面的下方，金屬網(wǎng)罩內(nèi)有一普通泡沫塑料片或無紡布，用以阻攔大顆粒塵埃，應(yīng)常檢查、拆洗，如發(fā)現(xiàn)泡沫塑料老化，要及時更換。除進(jìn)風(fēng)口以外，如有漏氣孔隙，應(yīng)當(dāng)堵嚴(yán)，如貼膠布，塞棉花，貼膠水紙等。工作臺正面的金屬網(wǎng)罩內(nèi)是超級濾清器，超級濾清器也可更換，如因使用年久，塵粒堵塞，風(fēng)速減小，不能保證無菌操作時，則可換上新的。
Clean Bench at the back or front air inlet below the metal mesh cover within a normal foam piece or non-woven fabrics used to block large particles of dust, should be a routine check, unpick and wash, such as found in foam aging, must be promptly replaced. In addition to outside air inlet, where gas leakage pores, it should be blocked strict, such as patching, plug of cotton and paste glue paper. Table inside the front cover of the metal mesh filter is super, super filter may be replaced, as a result of the use of long years, dust jam, wind speed decreased, can not guarantee aseptic operation, can be replaced with new.
超凈臺使用壽命的長短與空氣的潔凈程度有關(guān)。在溫帶地區(qū)超凈臺可在一般實驗室使用，然而在熱帶或亞熱帶地區(qū)，由于大氣中含有高量的花粉，或多粉塵的地區(qū)，超凈臺則宜放在較好的有雙道門的室內(nèi)使用。任何情況下不應(yīng)將超凈臺的進(jìn)風(fēng)罩對著開敞的門或窗，以免影響濾清器的使用壽命。
Clean Bench length of life related to the degree of air cleanliness. Clean Bench in temperate regions may be in the general laboratory use, but in tropical or subtropical regions, because the atmosphere contains high levels of pollen, dust or more areas, Clean Bench are advised to put a better interior double doors. Any case, Taiwan should not be ultra-clean air inlet hood facing the open door or window, so as not to affect the filter life.
無菌室應(yīng)定期用70%酒精或0.5%苯酚噴霧降塵和消毒，用2%新潔爾滅抹拭臺面和用具(70%酒精也可)，用福爾馬林(40%甲醛)加少量高錳酸鉀定期密閉熏蒸，配合紫外線滅菌燈(每次開啟15分鐘以上)等等消毒滅菌手段，以使無菌室經(jīng)常保持高度的無菌狀態(tài)。接種箱內(nèi)部也應(yīng)裝有紫外線燈，使用前開燈15分鐘以上照射滅菌，但凡是照射不到之處仍是有菌的。在紫外線燈開啟時間較長時，可激發(fā)空氣中的氧分子締合成臭氧分子，這種氣體成分有很強(qiáng)的殺菌作用，可以對紫外線沒有直接照到的角落產(chǎn)生滅菌效果。由于臭氧有礙健康，在進(jìn)入操作之前應(yīng)先關(guān)掉紫外線燈，關(guān)后十多分鐘即可入內(nèi)。
Sterile room should be on a regular basis with 70% alcohol or 0.5% phenol spray dust and disinfected with 2% wiped countertops and appliances Bromogeramine (70% alcohol can also be), with formalin (40% formaldehyde) plus a small amount of high manganese airtight fumigation potassium on a regular basis to meet the ultraviolet sterilization lamps (each to open more than 15 minutes) and so on means of disinfection and sterilization, sterile rooms in order to maintain regular high level of sterility. Vaccination should also be equipped with a UV box inside lights, turn on the lights more than 15 minutes prior to use radiation steril
原文：http://www.klcfilter.com/js_view.asp?id=33387
